Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: mRNA expression of matrix metalloproteinases (MMPs) 2, 9 (a) and tissue inhibitors of metalloproteinase (TIMPs) 1, 2 (b) in the placental tissue samples from pregnancies complicated by early-onset preeclampsia (EOPE). Bar diagrams represent the relative mRNA expression of MMPs 2, 9 (a) and TIMPs 1,2 (b) in placentae from EOPE and normotensive, non-proteinuric controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a positive control. Data presented as mean ± SEM. Wilcoxon matched-pairs signed rank (MMPs 2, 9, TIMP-1) and paired t (TIMP-2) tests were applied, *p≤0.05 was considered statistically significant, *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, ns: not significant.

Article Snippet: Overnight incubation was done with primary antibodies (MMP-2 (Abcam) at a dilution of 1:1000, MMP-9 (Abcam) at a dilution of 1:1000, TIMP-1 (Thermo) at a dilution of 1:200, TIMP-2 (Novus Biologicals) at a dilution of 1:200, CBS (Abcam) at a dilution of 1:1000 and Sp1 (Merck) at a dilution of 1:400] at 4°C.

Techniques: Expressing, Positive Control

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: Protein expression of matrix metalloproteinases (MMPs) 2, 9, and their feedback inhibitor tissue inhibitors of metalloproteinase (TIMPs) 1, 2 as analysed by immunohistochemistry (IHC). Representative IHC images of placentae from early-onset preeclampsia (EOPE) (a, e, i, m) and normotensive, non-proteinuric controls (b, f, j, n) showing MMP-2, MMP-9, TIMP-1 and TIMP-2 localization in syncytiotrophoblasts, stromal component, and blood vessels. Positive controls for MMP-2 (human placenta (c)), MMP-9 (human spleen (g)), TIMP-1 (rat brain (k)) and TIMP-2 (human pancreas (o)). Negative controls for MMP-2 (d), MMP-9 (h), TIMP-1 (l) and TIMP-2 (p), Scale Bar: 50 µm.

Article Snippet: Overnight incubation was done with primary antibodies (MMP-2 (Abcam) at a dilution of 1:1000, MMP-9 (Abcam) at a dilution of 1:1000, TIMP-1 (Thermo) at a dilution of 1:200, TIMP-2 (Novus Biologicals) at a dilution of 1:200, CBS (Abcam) at a dilution of 1:1000 and Sp1 (Merck) at a dilution of 1:400] at 4°C.

Techniques: Expressing, Immunohistochemistry

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: Protein expression of matrix metalloproteinases (MMPs) 2, 9 and tissue inhibitors of metalloproteinase (TIMPs) 1, 2 as analysed by immunofluorescence (IF) in EOPE and normotensive controls. Representative IF images of placentae from EOPE and normotensive, non-proteinuric controls showing MMP-2 (a, c (FITC stained), b, d (merged)], MMP-9 (e, g (FITC stained), f, h (merged)), TIMP-1 (i, k (TRITC stained), j, l (merged)) and TIMP-2 (m, o (FITC stained), n, p (merged)) localization in syncytiotrophoblasts, stromal component and blood vessels. Nuclei were stained by 4′,6-diamidino-2-phenylindole (DAPI); Scale Bar: 50 µm, FITC: Fluorescein isothiocyanate, TRITC: Tetramethylrhodamine isothiocyanate.

Article Snippet: Overnight incubation was done with primary antibodies (MMP-2 (Abcam) at a dilution of 1:1000, MMP-9 (Abcam) at a dilution of 1:1000, TIMP-1 (Thermo) at a dilution of 1:200, TIMP-2 (Novus Biologicals) at a dilution of 1:200, CBS (Abcam) at a dilution of 1:1000 and Sp1 (Merck) at a dilution of 1:400] at 4°C.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: Immunoblot of matrix metalloproteinases (MMPs) 2, 9 (a, b) and tissue inhibitors of metalloproteinase (TIMPs) 1, 2 (c, d) in the placentae from early-onset preeclampsia (EOPE) as compared to normotensive, non-proteinuric control placentae. Representative images of immunoblot showing the protein expression of MMP-2 (a), MMP-9 (b), TIMP-1 (c) and TIMP-2 (d) in placental tissues of EOPE and normotensive, non-proteinuric controls. Bar diagrams represent the normalized values of MMPs 2, 9 (e) and TIMPs 1, 2 (f) with respect to β-actin (loading control). Data presented as mean ± SEM. Statistical analysis was done using the Wilcoxon matched-pairs signed rank (MMPs 2, 9, TIMP-1) and paired t (TIMP-2) tests; *p≤0.05 was considered statistically significant. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, ns: not significant

Article Snippet: Overnight incubation was done with primary antibodies (MMP-2 (Abcam) at a dilution of 1:1000, MMP-9 (Abcam) at a dilution of 1:1000, TIMP-1 (Thermo) at a dilution of 1:200, TIMP-2 (Novus Biologicals) at a dilution of 1:200, CBS (Abcam) at a dilution of 1:1000 and Sp1 (Merck) at a dilution of 1:400] at 4°C.

Techniques: Western Blot, Control, Expressing